紫果猕猴桃幼胚愈伤组织诱导及植株再生

Callus Induction and Plantlet Regeneration from The Immature Embryo of Actinidia arguta var. purpurea

摘要: 以紫果猕猴桃(Actinidiaargutavar.purpurea)幼胚为外植体,诱导愈伤组织并进行植株再生。结果表明:不同的培养基和不同的培养条件对幼胚愈伤组织的诱导率及分化率不同;0.2mg/LZT与0.5mg/LGA₃配合使用有利于促进愈伤组织的诱导;7%蔗糖、600mg/LCH与400mg/LGln都有利于促进愈伤组织的形成;在添加0.5mg/L6-BA、0.05mg/LNAA与0.5mg/LGA₃的MS培养基中植株的再生率达93.3%。

Abstract: The immature embryo of Actinidia argutaTB var. purpurea were cultured on three different media to induce callus. The results show the callus was obtained from basic MS media with the combination of 0.2 mg/L ZT and 0.5 mg/L GA₃. High callus induction frequency was obtained from MS media supplemented with 7% surgar, 600 mg/L CH and 400 mg/L Gln. The highest rate of regeneration was found under the MS media supplemented with 0.5 mg/L 6-BA, 0.05 mg/L NAA and 0.5 mg/L GA₃.