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对几种百合科植物基因组大小的评价

DISCUSSION ON THE GENOME SIZE OF SEVERAL SPECIES OF LILIACEAE

  • 摘要: 利用孚尔根显微分光光度法测定了洋葱(Alliumcepa L.)、头(A.chinese G.Don,)、分葱(A.ascalonium L.)和黄花(Hemerocallis citrina Baroni)4种百合科植物根尖的细胞核DNA含量,并根据各自的核型得出了每个物种的基因组大小。它们分别为:洋葱18.16×109bp、头12.84×109bp、分葱10.44×l09bp和黄花7.48×109bp.由此认为,基因组是生物体维持其生存所需的全套基因。它反映了生物物种全部的、特定的遗传信息。与胞核DNA含量相比较,基因组大小更适合作为生物研究的特征参数。这不仅对细胞学、分类学和遗传学,而且对分子生物学亦有一定的意义。

     

    Abstract: The cell nuclear DNA contents in the root tip of several species of Liliaceae were measured by the Feulgen microspectrophotometry. The genome size of each species was calculated according to their nuclear types.They were as follows:A. cepa L.18. 16× 109 bp,A. chinese G. Don. 12. 84 ×10 bp,A.ascalonicum L. 10. 44 ×10 bp and H. citrina B. 7. 8 ×10 bp.The results suggested that the genome size should be more suitable to be used as a characteristic index of biological research than the nuclear DNAcontents, because the genome contained all genes which maintain the organisms' survival and refelected the entire specific genetic informations. This study will provide valuable reference not only to Cytology,Taxonomy and Genetics,but also to Molecular biology.

     

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