ULTRA-THIN SLAB IEF-PAGE METHOD——A EFFECTIVE BIO-ANALYTIC METHOD FOR ISOZYME ANALYSIS
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摘要: 介绍了一种简易、高效、快速的生物同工酶分析方法——超薄平板微型聚丙烯酰胺凝胶的等电聚焦电泳方法。该方法与目前国内外市场销售的同种用途等电聚焦的产品(如BIO-RAD公司的model 111 Mini IEF Cell)相比, 具有以下优点: 实验操作灵活, 用途更广;谱带清晰;每次可实验的样本量大;电泳所需时间短;实验费用低;适用于多种酶系统的同工酶和等位酶分析;凝胶干燥不需任何设备且能长期保存;样本用量少。Abstract: The paper introduces a simple, efficient and fast bio analytic method for isozyme analysis: ultra thin slab IEF PAGEsystem.In comparison with other commercial products (such as BIO RAD model 111 Mini IEFcell, etc.), the system has many advantages: (1)more flexibility in applications;(2)wider range of applications;(3)higher resolution separations;(4)larger capacity of running samples;(5)shorter time for electrophoresis;(6)lower cost;(7)suitable for various enzyme systems;and (8)no need for gel drying.
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Keywords:
- Gel electrophoresis /
- Isoelectriphoresis focusing /
- Isozymes
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