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Cd2+、Hg2+对菱幼苗生长及其超氧化物歧化酶、过氧化物酶活性的影响

EFFECTS OF Cd2+ ,Hg2+ ON GROWTH AND ACTIVITY OF SUPEROXIDE DISMUTASE AND PEROXIDASE IN TRAPA BISPINOSA ROXB.SEEDLINGS

  • 摘要: 利用不同浓度Cd2+、Hg2+处理菱幼苗,研究重金属离子对菱生长、超氧化物歧化酶(SOD)、过氧化物酶(POD)活性的影响,比较Cd2+、Hg2+对同一植物的毒性差异。Cd2+、Hg2+各处理浓度均抑制菱幼苗生长,使叶绿素含量下降,但Cd2+的抑制作用比Hg2+的作用明显。Cd2+、Hg2+对SOD、POD活性有不同的影响效果:Cd2+处理最初诱导SOD、POD活性升高,但随浓度加大时间延长酶活性急剧下降;Hg2+处理的酶活性变化相对平缓,其中5μmol/L和10μmol/LHg2+处理的POD活性持续上升。实验结果表明,在相同处理时间和浓度条件下,Cd2+比Hg2+对菱的毒性要大。依据实验结果,作者探讨了重金属对植物的毒害机制

     

    Abstract: Treating the Seedling of Trapa bispinosa using different concentrations of Cd2+ and Hg2+ ,the effects of Cd2+ and Hg2+ on growth and activity of superoxide dismutase (SOD) and peroxidase (POD) in the species were studied.All treatments with different concentrations Cd2+ 、Hg2+ can inhibit growth and reduce chlorophyll content of T.bispinosa seedlings,but the inhibition of Cd2+ are more significant than that of Hg2+ .Effects of Cd2+ and Hg2+ on activity of SOD and POD are different:treatment with Cd2+ can initially induce the activity of SOD and POD to rise,but with extending time and increasing concentration,the activity declines sharply;while activity of SOD and POD treated with Hg2+ relatively changes smoothly,in which activity of POD treated with 5 μmol/L and 10 μmol/L Hg2+ rises continually.Experimental results indicate that toxicity of Cd2+ on T.bispinosa seedlings is heavier than that of Hg2+ under the same treating time and concentration.According to the results,authors try to inquire into the toxicity mechanism of heavy metal on plants.

     

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