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Slr1122影响Hik12磷酸化并参与调节Synechocystis sp.PCC 6803的色素合成

李想, 董亮亮, 张伦, 赵开弘, 周明

李想, 董亮亮, 张伦, 赵开弘, 周明. Slr1122影响Hik12磷酸化并参与调节Synechocystis sp.PCC 6803的色素合成[J]. 植物科学学报, 2013, 31(3): 248-260. DOI: 10.3724/SP.J.1142.2013.30248
引用本文: 李想, 董亮亮, 张伦, 赵开弘, 周明. Slr1122影响Hik12磷酸化并参与调节Synechocystis sp.PCC 6803的色素合成[J]. 植物科学学报, 2013, 31(3): 248-260. DOI: 10.3724/SP.J.1142.2013.30248
LI Xiang, DONG Liang-Liang, ZHANG Lun, ZHAO Kai-Hong, ZHOU Ming. Slr1122 Affects Activity of Hybrid Sensory Kinase and Regulates the Biosynthesis of Carotenoids in Synechocystis sp.PCC 6803[J]. Plant Science Journal, 2013, 31(3): 248-260. DOI: 10.3724/SP.J.1142.2013.30248
Citation: LI Xiang, DONG Liang-Liang, ZHANG Lun, ZHAO Kai-Hong, ZHOU Ming. Slr1122 Affects Activity of Hybrid Sensory Kinase and Regulates the Biosynthesis of Carotenoids in Synechocystis sp.PCC 6803[J]. Plant Science Journal, 2013, 31(3): 248-260. DOI: 10.3724/SP.J.1142.2013.30248
李想, 董亮亮, 张伦, 赵开弘, 周明. Slr1122影响Hik12磷酸化并参与调节Synechocystis sp.PCC 6803的色素合成[J]. 植物科学学报, 2013, 31(3): 248-260. CSTR: 32231.14.SP.J.1142.2013.30248
引用本文: 李想, 董亮亮, 张伦, 赵开弘, 周明. Slr1122影响Hik12磷酸化并参与调节Synechocystis sp.PCC 6803的色素合成[J]. 植物科学学报, 2013, 31(3): 248-260. CSTR: 32231.14.SP.J.1142.2013.30248
LI Xiang, DONG Liang-Liang, ZHANG Lun, ZHAO Kai-Hong, ZHOU Ming. Slr1122 Affects Activity of Hybrid Sensory Kinase and Regulates the Biosynthesis of Carotenoids in Synechocystis sp.PCC 6803[J]. Plant Science Journal, 2013, 31(3): 248-260. CSTR: 32231.14.SP.J.1142.2013.30248
Citation: LI Xiang, DONG Liang-Liang, ZHANG Lun, ZHAO Kai-Hong, ZHOU Ming. Slr1122 Affects Activity of Hybrid Sensory Kinase and Regulates the Biosynthesis of Carotenoids in Synechocystis sp.PCC 6803[J]. Plant Science Journal, 2013, 31(3): 248-260. CSTR: 32231.14.SP.J.1142.2013.30248

Slr1122影响Hik12磷酸化并参与调节Synechocystis sp.PCC 6803的色素合成

基金项目: 国家自然科学基金资助项目(31070743,21072068)。
详细信息
    作者简介:

    李想(1987- ),女,硕士研究生,研究方向为蓝细菌光合作用与蛋白质工程。

    通讯作者:

    周明, E-mail: zhouming321@126.com

  • 中图分类号: Q933

Slr1122 Affects Activity of Hybrid Sensory Kinase and Regulates the Biosynthesis of Carotenoids in Synechocystis sp.PCC 6803

  • 摘要: Synechocystis sp.PCC 6803是一种良好的研究光合作用的模式生物,其中slr1122编码一个250个氨基酸的未知蛋白。据报道Slr1122可能与杂合传感激酶(hybrid sensory kinase)Sll1672(Hik12)相互作用,本研究通过复合物实验证实了Slr1122与Sll1672确实存在相互作用。利用 32P标记证明,在加入Slr1122后Hik12的磷酸化受到了明显的影响,推测其可能参与该双组分系统的调控。通过同源双交换,用卡那霉素抗性基因替换slr1122,将slr1122Synechocystis sp.PCC 6803中敲除,构建了slr1122的缺失体Δslr1122。研究发现在Δslr1122中,编码PSⅡ中核心蛋白D1亚基的slr1181(psbAI)的转录水平明显降低,使PSⅡ光合作用受到影响,导致Δslr1122的生长速率低于野生型(WT)。同时slr1122的缺失使得蓝细菌对光的敏感性增强,在弱光条件下,Δslr1122对光能的利用效率高于WT,其生长速率也较WT高,但与此相反,Δslr1122对强光的耐受力及生长速率则不及WT。Δslr1122体内的藻胆蛋白含量与色素含量均降低,尤其是类胡萝卜素,RT-PCR的结果也显示合成类胡萝卜素过程中的5个关键酶转录水平均下降。这可能是Δslr1122对氧化胁迫变得敏感的原因之一。总之,Slr1122影响杂合传感激酶Hik12磷酸化并参与调节Synechocystis sp.PCC 6803的光合色素合成。
    Abstract: Synechocystis sp.PCC 6803 is suitable for studies of photosynthesis.Further,slr1122 encodes an unknown protein of 250aa,which possibly interacts with a hybrid sensory kinase encoded by sll1672 (Hik12).Via pull-down assay, we verified that Slr1122 interacted with Sll1672.With the isotope assay of 32P,the auto-phosphorylation of Hik12 decreased in the presence of Slr1122,indicating that Slr1122 regulated the two-component system.By homologous double-crossover,slr1122 was replaced with antibiotic resistance cassettes kanamycin,resulting in the knockout mutant of Synechocystis sp.PCC 6803,Δslr1122.We found that in Δslr1122,the transcription of the key PSⅡ component gene,psbAI (slr1181),decreased remarkably,leading to low capacity of photosynthesis and low growth rate of Δslr1122 compared with the wild type.In addition,Δslr1122 became more sensitive to light.Under low light,Δslr1122 caught more light and grew more rapidly,with the opposite occurring under high light,and thus Δslr1122 was more liable to photodamage.Δslr1122 had lower content of phycobiliprotein and photosynthetic pigments,particularly carotenoids.RT-PCR showed that the five important genes involved in carotenoid biosynthesis were transcribed more lowly.Coincident with this,Δslr1122 was more sensitive to redox stress and high light stress.In conclusion,Slr1122 was involved in the regulation of phosphorylation of Hik12 and thereby influenced the biosynthesis of carotenoids.
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出版历程
  • 收稿日期:  2012-12-20
  • 修回日期:  2013-03-24
  • 发布日期:  2013-06-29

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