Tagging and Isolation of the Anther-specific Promoter in Hexaploid Tritordeum

The promoter is an important element of gene expression,and tissue specific promoters are very useful in gene engineering.A promoterless UidA containing plasmid derived from pPLGUS was transformed into tritordeum.Histochemical analysis of various tissues in transgenic tritordeum was carried to examine tissue specific expression of GUS activity.A transformant line with anther-specific GUS expression was successfully labeled and isolated.PCR method was used to isolate this anther-specific promoter with DNA extracted from leave as template,using primer pair of rice anther specific promoter,primer P1 as forward primer,UidA gene specific sequence as reverse primer.By sequencing and analyzing the amplified DNA fragment from PCR reaction,a part of UidA gene and a flanking sequence were obtained.Some essential promoter elements were found in this sequence.Therefore the fragment obtained was believed to be a part of anther specific promoter.