Bioinformatics and Expression Pathogenesis-related Protein 10 Gene(SmPR-10) from Salvia miltiorrhiza Bunge

After analysis of Salvia miltiorrhiza EST sequences,one sequence was found had high homology with pathogenesis-related protein 10.The cDNA and DNA sequence of the gene were cloned and named as SmPR-10(GenBank accession number:HM439764).SmPR-10 without intron contained a single opening reading frame of 486 bp encoding 161 amino acid-peptides.SmPR-10 had a Bet v1 domain and was classed into the pathogenesis-related protein 10 family according to its sequence.Bioinformatics showed that the putative protein was a stable acidic protein with a predicted molecular weight of 17.47 kD.There were no signal peptides and transmembrane domains in SmPR-10.The Quantitative RT-PCR results revealed that SmPR-10 was expressed in different organs of S.miltiorrhiza,and could be induced by methyl jasmonate and pathogen.This suggested that SmPR-10 may be involved in plant defenses.