Wild Rice Gene Expression in Response to Brown Planthopper (Nilaparvata lugens Stål) Feeding and Whitebacked Planthopper (Sogatella furcifera Horvath) Feeding

Brown planthopper (Nilaparvata lugens (Stål),BPH) and whitebacked planthopper (Sogatella furcifera Horvath,WBPH) are the phloem-sucking herbivores of rice.We employed cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique to characterize the mole-(cular) responses of wild rice Oryza officinalis Wall ex Watt to BPH and WBPH feeding.After investigating about 7% of the insects-responsive transcriptome,we isolated 258 differential transcript-derived fragments (TDFs) and sequenced 112,of which 44 presented similarities to known or putative functional proteins.Among these 44 TDFs,35 TDFs were altered by WBPH-feeding while 24 were by BPH-feeding.There were more down-regulated TDFs than the up-regulated ones found after fed by BPH and WBPH.Only 2 TDFs were co-induced but 12 were co-suppressed by two planthoppers feeding.The results revealed that O.officinalis responded differentially to these two phloem herbivores.