Study on Extractive Craft of Polysaccharides by Enzymolysis with Watering from Ziziphus jujuba Mill.in the north of Shaanxi
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Abstract
Taking polysaccharide content and extract rate as the index,the orthogonal was used for optimizing extraction and isolation procedure of polysaccharide from Ziziphus jujuba Mill.in the north of Shaanxi.The result shown that extraction employed enzymolysis with watering was the best one.(1) Temperature 70℃,enzyme recruitment 3.0%,2 hours;(2) Temperature 70℃,enzyme recruitment 2.0%,4 hours.When fractionated precipitation of polysaccharide,the optimize ratio between concentrated solution and absolute ethyl alcohol is 1∶3,while de-colorization,the amount of activated charcoal is 7 g/L.
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