Callus Induction,Differentiation and Establishment of Plantlet Regeneration System of Salsola ferganica

The effects of explants and plant growth regulators on callus induction,differentiation and plantlet regeneration of Salsola ferganica Drob.were studied.The results showed that the best medium of callus induction was MS medium with 2,4-D 2.0 mg/L+6-BA 0.2 mg/L.Hypocotyl was the best explant.The best medium for the subculture of callus was MS+NAA 0.1 mg/L+6-BA 1.0 mg/L.The MS me-dium with 6-BA 0.5 mg/L+NAA 0.05 mg/L was preferable for inducing adventitious shoot.The 1/2 MS medium with IBA 0.2 mg/L was suitable for the root differentiation and the rate of rooting was 72%.The best medium for inducing adventitious shoot of cotyledon with petiole was MS+6-BA 1.0 mg/L and the shoot regeneration frequency was 90.74%.