ORGANOGENESIS FROM IN VITRO CULTURE OF YOUNG COCONUT INFLORESCENCE

Callus was induced in the modified MShasal medium with high concentration of NAAand 2,4-Dfrom young inflorescence of coconut (Cocos nucifera L.).During the callus subculture with gradually reduced concentration of NAAand 2,4-D,it developed into compact nodular tissue.There were conducting tissue,meristematic cell clumps,root primordia and embryoids at different developing stage in the nodular tissue.The normal roots and green buds were developed subsequently from some of the nodular tissue.At the same time,some green buds grew out directly from the base of the young flower branches, and finally became the plantlets with normal roots and leaves,but without apical meristem and leaf primordia.Moreover,some of the stem apex developed into flower branches and even showed several small abnormal flowers.