ESTABLISHMENT AND SUSTAINATION OF EMBRYOGENIC SUSPENSION CULTURE OF WHEAT (TRITICUM AESTIVUM L.)

To establish excellent embryogenic suspension culture is the key step of protoplast culture of grasses. We describe a way of establishment and sustaination of embryogenic suspension culture with high plant regenerability of wheat. Fast growing ,fine dispersed embryogenic suspension cultures with high plant regenerability were established within 2 weeks when type Ⅱ embryogenic calli were cultured in an improved liquid MSmedium (SDL) with approximate 3 days doubling time; Ameliorated N₆ liquid medium (NBDL) did not support embryogenic suspension cultures very seccessfully with approximate 5 days doubling time ; AAliquid medium was failed to support embryogenic suspension cultures of wheat. Plant regenerability of embryogenic suspension cultures was declined when they were cultured for a long period of time,but it was restored when the cultures were transplanted to solid NBDmedium previously for a short time and then transferred to liquid SDLmedium. We sustained the regenerability successfully by using this approach.