CULTURE AND PLANT REGENERATION OF ACTINIDIA DELICIOSA PROTOPLASTS ISOLATED FROM COTYLEDON CALLUS

Callus lines of A₁₁B₂ and A₁₆N₁ were selected from cotyledon cultures on B₅ and NN-69 media containing 1mg/L 2,4-D, respectively. When protoplasts isolated from callus line A₁₁B₂ were cultured on the modified B₅ medium, cell division was arrested at the stage of cell clusters. However, when protoplasts isolated from callus line A₁₆N₁ were cultured on the modified NN-69 medium, they had divided to form cell groups, then developed into callus, from which plants were regenerated by the method of the stepwise induction of organgenesis.