Study on HPLC Finger Print of Chinese Quince

The chromatogram fingerprint of the compound Chaenomeles speciosa(Sweet) Nakai was established by HPLC.Analysis was performed on a YPW-Kromasil TM-C18 analytical column(250 mm×4.6 mm,5 μm) with methanol(A) and 1% glacial acetic acid(B) as mobile phases in gradient mode.The column temperature was set at 30℃ and the flow rate was 1.0 mL/min with the detection wavelength of 290 nm.By analyzing the fingerprint chromatogram,Chaenomeles speciosa showed 11 characteristic peaks established from 19 specimens.The similarity of each specimen was good.The accuracy,repeatability,and stability of this method were satisfied,the RSDs of relative retention time and area of aimed peaks existed in all samples.Results showed that the chromatographic fingerprint of Chaenomeles speciosa with high characteristics and specificity can be used as theories dependence to control its quality.