Taxonomic Study on a Taxol Producing Fungus Isolated from Bark of Taxus chinensis var.mairei

Taxonomic studies were preformed on the strain TPF6,which was isolated from the bark of Taxus chinensis var.mairei growing in Hubei Province.Phenotypic analysis and biolog analysis indicated that strain TPF6 show high similarity with genus Alternaria.Phylogenetic analysis based on 18S rDNA gene sequence indicated that the strain belongs to the genus Alternaria,sequence similarity values with its nearest neighbors are between 95%~99%,and share more than 99% with strain A. alternata.The predominant fatty acid components are 18:2 CIS9,12/18:0a and shares 59.8% the same cellular fatty acid composition with other members in Alternaria.On the base of taxonomic data,the strain was named as A. alternata.The taxol producing ability was determined by HPLC and MS.The result shown that the taxol concentration in fermentation medium is 84.5 μg/L.