Comparative Analysis of Oryza latifolia Genome with C₀t-1 DNA and Genomic DNA of Oryza sativa

C₀t-1 DNA and genomic DNA(gDNA) of cultivated rice Oryza sativa were used as probes to analyze comparatively the Oryza latifolia genome by fluorescence in situ hybridization(FISH).Results showed that the hybridization signals of gDNA were more distinct on the chromosomes of O.latifolia than that of C₀t-1 DNA.These hybridization signals were mostly distributed on centromeres and telomeres regions as well as those near centromeres under different stringencies.With increasing washing stringency,hybridization signals showed rela-tively higher species’ specificity.Karyotype analysis of O.latifolia was carried out according to fluorescence in situ hybridization results in different washing stringencies.This study can help improve correct identification of Oryza chromosomes.