Study on Subcellular Localization of Two Expressed Proteins in Arabidopsis thaliana

The information of protein subcellular localization is important to our understanding of a protein function.The proteins encoded by both At4g16410 and At1g18060 were predicted to be the Arabidopsis chloroplast proteins.The 199 bp and 220 bp fragments of this two genes at the 5’ end were cloned and fused with GFP to construct the binary vectors pMON530-cTP1-GFP and pMON530-cTP2-GFP for genetic transformation,respectively.Observed by a confocal laser-scanning microscopy,green fluorescent signals were localized in chloroplasts in transgenic Arabidopsis plants suggesting the 199 bp and 220 bp fragments encoding peptides targeted to chloroplast.The results suggest that At4g16410 and At1g18060 encode two chloroplast proteins.