A Preliminary Study on Interspecific Hybridization between Brassica napus and Purple-caitai

The embryo rescue technique was applied to obtain interspecific hybrids between Brassica napus and Purple-caitai.Of the media studied,MS supplemented with(1.0-2.0)mg·L^-1 6-BA+0.05 mg·L^-1 NAA+0.5% activated carbon+30 g·L^-1 sugar+7.5 g·L^-1 agar gave the most satisfactory results for ovary culture.The effect of activated carbon is more significant than that of medium and plant hormone in ovary culture.Ovaries taken 9,12,15 and 18 days after pollination were used for culture,and those taken 18 days after pollination appeared most efficient for seed production,that of 15 days takes second place.But the results by studying the germination of their hybrid seeds showed the germination rate of the ovary cultured hybrids taken 15 days after pollination is highest,namely 57.03%,the lowest in 18 days,Only 38.49%.