Advance Search
CHEN Ling, DING Li-Ping, YAN Ying-Jun, JIN Lian, YANG Guang-Xiao, TU Zhi-Ming, HE Guang-Yuan. Isolation and Sequence Analysis of TaPSG719 Gene Promoter from Wheat(Triticum aestivum L.)[J]. Plant Science Journal, 2009, 27(4): 361-364.
Citation: CHEN Ling, DING Li-Ping, YAN Ying-Jun, JIN Lian, YANG Guang-Xiao, TU Zhi-Ming, HE Guang-Yuan. Isolation and Sequence Analysis of TaPSG719 Gene Promoter from Wheat(Triticum aestivum L.)[J]. Plant Science Journal, 2009, 27(4): 361-364.
shu

Isolation and Sequence Analysis of TaPSG719 Gene Promoter from Wheat(Triticum aestivum L.)

  • China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, The Genetic Engineering International CooperationBase of Ministry of Science and Technology, the Key Laboratory of Molecular Biophysics of Ministry of Education, College of LifeScience and Technology, Huazhong University of Science & Technology(HUST), Wuhan 430074, China

More Information
  • Received Date: December 07, 2008
  • Revised Date: April 04, 2009
  • Published Date: August 20, 2009
    Graphical Abstract
    • Abstract
  • TaPSG719 gene is a pollen-specific gene isolated from wheat and its function is remains unknown.The aims of the study were to isolate the TaPSG719 promoter sequence and to characterize the promoter regions responsible for tissue-specificity.A pair of special primers was designed according to the cDNA sequence of TaPSG719 gene reported.The upstream regulatory sequence was cloned from total DNA of wheat(Bobwhite)by inverse-PCR.A fragment of 1776 base pairs was obtained by two rounds of inverse-PCR.Sequence analysis of functional elements by blasting PLACE and PlantCARE databases revealed that it contains TATA-box,CAAT-box,two pollen-specific elements(AGAAA and GTGA),several light responsive elements and hormone responsive elements.Promoter analysis of this gene would definitely help us to understand its function as well as the molecular biology of wheat male gametophyte development.
    • FullText(HTML)
    • References (0)
    • Related Articles

  • Related Articles

    [1]Li Rong, Sui Meng-Jie, Wang Qi-Gang, Yu Chun-Xia, Du Guang-Hui, Yan Hui-Jun. Cloning and analysis of RcAG promoter in Rosa chinensis ‘Viridiflora’[J]. Plant Science Journal, 2021, 39(4): 407-414. DOI: 10.11913/PSJ.2095-0837.2021.40407
    [2]Li Si-Yu, Yuan Yi-Jun, Zheng Yu-Sheng, Li Dong-Dong. Cloning and tissue-specific expression analysis of type2 diacylglycerol acyltransferase gene (DGAT2) promoter in Elaeis guineensis[J]. Plant Science Journal, 2018, 36(6): 835-841. DOI: 10.11913/PSJ.2095-0837.2018.60835
    [3]Zhao Xue-Rong, Yang Yan, Luo Xiao-Peng, Yao Pan-Feng, Wang An-Hu, Zhao Hai-Xia, Wu Qi. Cloning and analysis of chalcone synthase gene FtCHS1 promoter in Fagopyrum tataricum Gaertn.[J]. Plant Science Journal, 2017, 35(4): 543-550. DOI: 10.11913/PSJ.2095-0837.2017.40543
    [4]GUO Xiao-Rong, YANG Xin-Bing, WANG Huai-Qin, MING Fang-Lin, SHE Xu, CAO Xiao-Yan. Cloning and Expression Analysis of miR408 Precursor Sequences from Salvia miltiorrhiza[J]. Plant Science Journal, 2016, 34(3): 430-438. DOI: 10.11913/PSJ.2095-0837.2016.30430
    [5]ZHAO Jing, CAI Shen-Wen, XU Zhong-Rui, XIONG Zhi-Ting. Isolation and Activity Analysis of Cell Wall Invertase Gene Promoter (EhcwINVP) from Elsholtzia haichowensis Sun[J]. Plant Science Journal, 2016, 34(3): 420-429. DOI: 10.11913/PSJ.2095-0837.2016.30420
    [6]YANG Xiao-Hui, ZHAO Xue-Li, GAO Xin-Fen. Morphological Variation and ITS Sequence Analysis of the Indigofera szechuensis Complex[J]. Plant Science Journal, 2015, 33(6): 727-733. DOI: 10.11913/PSJ.2095-0837.2015.60727
    [7]WANG Shi-Ying, WANG Hao-Ru, WANG Jian. Functional Deletion Analysis of FaChit1 Promoter Region from Festuca arundinacea[J]. Plant Science Journal, 2013, 31(6): 562-569. DOI: 10.3724/SP.J.1142.2013.60562
    [8]CHEN Ling, SU Pei-Pei, TONG Han-Wen, LIU Yi-Ke, ZHU Zhan-Wang, YANG Guang-Xiao, GAO Chun-Bao, HE Guang-Yuan. Isolation of the Promoter Region of a Pollen-specific Gene PSG076 by Inverse-PCR[J]. Plant Science Journal, 2012, (3): 309-314. DOI: 10.3724/SP.J.1142.2012.30309
    [9]ZHANG Da-Le, LI Yu-Ge, ZHANG Xiao-Ping, SU Ya-Rui, LI Suo-Ping, WANG He-Zhou. Cloning and Sequence Analysis of α-gliadin Gene from Aegilops tauschii×Secale cereale Amphiploids[J]. Plant Science Journal, 2012, (3): 269-276. DOI: 10.3724/SP.J.1142.2012.30269
    [10]WANG Yao, HUANG Hui-Zhen, ZHANG Yong, YANG Bao-Yu, CHEN Shi-Yun. Isolation and Sequence Analysis of MARs from Populus nigra[J]. Plant Science Journal, 2007, 25(1): 24-28.

Catalog

    Get Citation
    PDF
    XML
    Article views (3581) PDF downloads (2038) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    Related Articles
    Copyright © 2022 Plant Science Journal 鄂ICP备05004779号-3
    Address：No. 201, Jiufeng 1st Road, Donghu High tech Zone, WuhanPostal Code：430074

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return