rDNA ITS Sequence Analysis of Different Cultivar Variety of Cornus officinalis

To study rDNA ITS sequence of Comus officinalis Sieb. Et. Zucc., and analyzed the rDNA ITS sequence of its different cultivated varieties. A pair of primers was selected by experi-ment. The ITS gene fragment was amplified using this pair of primers, and PCR products were purified with DNA gel extraction kit (AxyPrep~(TM)) and sequenced by the method of Sanger dideoxy. Then the ITS1-5. 8S-ITS2 sequences were analyzed by the software of DNAssist Ver-sion 2. 0. The ITS1-5. 8S-ITS2 sequences was found whole ITS sequence consisting of 682 bp, ITS1 253 bp,5.8S 156 bp and ITS2 273 bp. The 5.8S gene sequences of 7 fruit shapes show highly coincidence. The ITS1 and ITS2 gene sequences of cylinderic shape, long pear-shape, el-liptic shape and spindly shape are in coincidence. The short cylinderic shape have 1 variable site in 3’end of ITS1 and 5’end of ITS2. The short pear-shape have 3 variable site in 5’end of ITS1 and the long clinderic shape have 5 variable site in ITS1. The ITS1 and ITS2 gene fragments are conservative at specific level in C. Officinalis. While they are less difference among some culti-vars. So they can being molecular marker for authentication of C. Officinalis Sieb. Et. Zucc.