Tissue Culture of Male and Female Trichosanthes kirilowii Maxim

The results of tissue culture of male and female Trichosanthes kirilowii Maxim showed that there is difference of phytohormone concentration between male and female T.kirilowii.The suitable medium for the callus induction of female T.kirilowii is the medium MS+NAA 0.5 mg/L+IBA 0.5 mg/L+6-BA 1.5 mg/L.The suitable medium for the callus induction of male T.kirilowii is the medium MS+NAA 0.1 mg/L+IBA 0.5 mg/L+6-BA 0.5 m/L and MS+NAA 0.1 mg/L+IBA 1.0 mg/L+6-BA 1.0 mg/L.The suitable medium for the callus differentiation of female T.kirilowii is the medium MS+NAA 0.1 mg/L+IBA 0.4 mg/L+6-BA 1.2 mg/L.The suitable medium for the callus differentiation to male T.kirilowii is the medium MS+NAA 0.3 mg/L+IBA 0.2 mg/L+6-BA 0.8 mg/L.The suitable medium for rooting to male and female T.kirilowii free seedlings are both MS+NAA 0.1 mg/L.