Induction of Autotetraploid Non-heading Chinese Cabbage with High-quality and Heat-tolerance by Colchicine

Diploid plants of non-heading Chinese cabbage(Brassica campestris ssp.chinensis Makino)were induced by treating the apical portion of seedlings with different solution of colchicine.Autotetraploid plants were selected and identified based on morphological,anatomic,agronomic and cytological characters and nutritional traits.The results indicated that the treatment with 0.1 mol/L colchicine for(6 times)yielded the highest doubled chromosome at highest rates.Compared with the diploid plant,the autotetraploid plants possessed gigantism in plant,stomata,flowering organs.The density of stomata and fecundity were recede remarkably.The contents of protein,solubility sugar and vitamin C of the autotetraploid plants were increased by 15.69%,71.25% and 22.18% than that of diploid,respectively.The autotetraploid plants performed high-fertility and heat-tolerance under high temperature in summer.