Comparisons of Antioxidant Activity of the Polysaccharide from Different Parts of Elaeagnus umbellata Thunb.

Polysaccharide of branches,leaves,and seeds of Elaeagnus umbellata Thunb.were extracted with hot water.Protein was excluded using the savage method,and then concentrated and precipitated with 80% ethanol to obtain the polysaccharide.Antioxidant activity of polysaccharide was evaluated by the efficiency of scavenging the hydroxyl radical and superoxide anion radical and reductive potential,with ascorbic acid used as a positive control.Results showed that the polysaccharide with three parts was effective in removing hydroxyl radical,superoxide free radicals,the scavenging activity on hydroxyl radical from the highest to the lowest was seeds>leaves>branches>Vc,while superoxide removal activity from the highest to the lowest was seeds>Vc>leaves>branches.All of polysaccharides possessed strong reducing power,but the effect was not as Thiourea.