The Peroxidase Isoenzymes of Suspending Culture Cells of Cotton Induced by the Oligosaccharins from Verticillium dahliae

The living cell culture of cotton or its homogenates from V.dahliae-susceptible variety (S) or resistant variety (R) were added into the Czapeks liquid media which had been inoculated 3 d with the higher virulent strain(V44) and lower virulent strain(V64) of V.dahliae respectively.After 4 d additional incubation,the spore of these fungus were collected each to evaluate their wilting or virulence to cotton plants,and the cell wall of the hypha were used to prepare oligosaccharins(H,from V44 and L,from V64)which were as elicitors to induce the cell cultures of cotton.(Sor R) The PAGEspectrums of PODisozymes from cotton cells showed that (1) all oligosaccharins induced cotton cells to produce new PODisozymes; (2)the living cotton cells or its homogenate from susceptible variety affected strongly the fungus.The cotton PODspectrum at 36 h after induction showed that in all the inducing systems,the most outstanding diversity is between S→L(H)→Sand their control,i.e.L(H)→S,i.e.the oligosaccharins from the fungi contacted with the living cotton cells or its homogenate from susceptible variety induced the most strong change of PODisozymes in the living cotton cells from susceptible variety,as compared with the control,L(H)→S.The wilting result showed the living cotton cells from susceptible variety increased the virulence and pathogenicity of the fungi; (3)the impact of living cotton cells or its homogenate from resistant variety on the fungi is different from susceptible one.There is no change of enzyme bands,but the new enzymes occurred at 9 h in H→Rand 24 h in H→Safter induction were ahead to 6 h in R→H→Rand 9 h in R→H→S.The wilting result showed the living cotton cells from resistant variety had little influence on the virulence and pathogenicity of the fungi.