Identification of Hybrid of <I>Oryza sativa×O.meyeriana</I> Using Genomic in situ Hybridization

JIA Dong-Liang; SHU Li-Hui; SONG Yun-Chun; QIN Rui; ZHU Li-Li; HE Guang-Cun

JIA Dong-Liang, SHU Li-Hui, SONG Yun-Chun, QIN Rui, ZHU Li-Li, HE Guang-Cun. Identification of Hybrid of Oryza sativa×O.meyeriana Using Genomic in situ Hybridization[J]. Plant Science Journal, 2001, 19(3): 177-180.

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Identification of Hybrid of Oryza sativa×O.meyeriana Using Genomic in situ Hybridization

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With biotin-labelled total genomic DNAs from Chinese wild rice, Oryza meyeriana as probe and 4-fold unlabelled total genomic DNA from cultivated rice,O.sativa IR 36 as blocking,genomic in situ hybridization was applied to somatic chromosome preparations of O.sativa×O.meyeriana F_{1_{hybrid.When detected with anti-biotin-FITC,O.meyeriana chromosomes gave gree-nish-yellow fluorescence while cultivated rice chromosomes fluoresced red with PIcounterstain.The area of signal on each wild chromosome was small.It suggested that a great part of DNA sequence in O.meyeriana is homologous to cultivated rice.}}

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Identification of Hybrid of Oryza sativa×O.meyeriana Using Genomic in situ Hybridization

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