A Study on Asymbictic Germination of Phalaenopsis

Seeds asymbictic germination of Phalaenopsis ’Fun Time’ was studied.On the KC, (1/2 MS) and Hyponex No.1 basal media supplemented with various complex additives(i.e. (banana) juice, apple juice, tryptone and active carbon)seeds germinated well.Exogenous plant growth substances inhibited seeds germination.9 g·L^-1 Agar and pH value(5.2-5.4)were favourite for germination. The germination percentage of seeds aged 120 days after pollination was over 85%. Three media such as KC, 1/2 MS, and revised NITSCH supplemented with (1 mg·L^-1) KT+0.1 mg·L^-1 2,4-D were used to induce PLB, supplemented with 1 mg·L^-1 NAA +1-3 mg·L^-1 KT or 3-5 mg·L^-1 BA were used to multiply PLB . Hyponex No.1 or (1/2 MS) medium supplemented with banana juice, tryptone, malic acid and active carbon were suitable for root organogenesis and seedlings growth.