Construction of Plant Expression Vector Harbouring Bivalent Genes Composed of Synthetic VHb Gene and GFMcryIA Gene

The highly-efficient plant expression vector harbouring bivalent genes was constructed by applying the artificially-modified-synthesized VHb gene(vgbM gene)and the modified Bt gene(GFMcryIA gene),vgbM gene experession cassettes contained such cis-acting elements as 35S promoter,two enhancers,Ω and Kozak sequence,poly-terminator and Nos terminator,besides these elements,Bt gene expression cassettes contained splicing sequence,processing sequence and Poly(A)sequence.Transgenic tobacco plants were obtained through the transformation with Agrobacterium tumefaiens carrying the expression vector PGBI4ASVHBBt,by PCR amplification and Southern blot analysis,it was proved that the bivalent genes were inserted into the tobacco plant genome;through western blot analysis it was also verified that vgbM gene expressed in the transgenic plants.Toxicity assay indicated that transgenic plants expressed pesticidal toxin protein.