Preliminary Function Analysis of Rice OsRab7 in Salt Tolerance and Construction of Its Genetic Transformation Vector

Rab7 is a small GTP-binding protein belonging to the Rab family,which plays an important role in vesicle trafficking,fusion and membrane organization.Total RNA was extracted from the young leaves of ‘Nipponbare’,a salt sensitive rice variety(Oryza sativa L ssp.japonica)and specific PCR primers were designed.The sequence of OsRab7 was obtained by RT-PCR to construct the pET-32a-OsRab7 vector.Escherichina coli was used as a host organism to express and test the function of the protein in salt tolerance.The experiment has demonstrated that OsRab7 has largely improved the salt tolerance of E.coli in medium with high NaCl concentration(4.5%-8.5%).The plant genetic transformation vector was constructed using p1301B(a pCAMBIA1301 variation)and rice callus from ‘Zhonghua 11’(salt sensitive)was transformed via Agro-bacteria transformation.The expressed protein was purified as well for further research.