THE CYTOLOGICAL OBSERVATION OF MICROSPORE EMBRYOGENESIS IN BRASSICA NAPUS

The cytological observation of microspore embryogenesis of Brassica napus DH line Bac 604 has been shown that microspores survival rate decreased rapidly as daysof culture increased in l～5 days culture. The microspores with embryogenesis swelt apparenty after 2～3 days culture. Some of the swollen microspores began to divide into 2cell, 3-cell with "(这路有图片19980302-197-1.GIF)"shaped, multicellular proembryos in order, which further developedinto globular with attached suspensor, hearted, and torpdo embryoids successively. Generally,the attached suspensor was divorced from the main part of the embrybid into liquid medium during the hearted-shaped embryoid. The division did not appear in most ofthe swollen cells, or after some of cells began to divide for one to several times,theyceased development.