Gong Xiaosong, Zeng Fuhua, Yan Longfei. AN EFFICENT METHOD FOR THE PURIFICATION OF CHLOROPLAST DNA FROM HIGHER PLANTS[J]. Plant Science Journal, 1994, 12(3): 280-280.
Citation:
Gong Xiaosong, Zeng Fuhua, Yan Longfei. AN EFFICENT METHOD FOR THE PURIFICATION OF CHLOROPLAST DNA FROM HIGHER PLANTS[J]. Plant Science Journal, 1994, 12(3): 280-280.
Gong Xiaosong, Zeng Fuhua, Yan Longfei. AN EFFICENT METHOD FOR THE PURIFICATION OF CHLOROPLAST DNA FROM HIGHER PLANTS[J]. Plant Science Journal, 1994, 12(3): 280-280.
Citation:
Gong Xiaosong, Zeng Fuhua, Yan Longfei. AN EFFICENT METHOD FOR THE PURIFICATION OF CHLOROPLAST DNA FROM HIGHER PLANTS[J]. Plant Science Journal, 1994, 12(3): 280-280.
AN EFFICENT METHOD FOR THE PURIFICATION OF CHLOROPLAST DNA FROM HIGHER PLANTS
The method developed by Bookjans et al.(1984)for chloroplast DNA(ctDNA) purification was improved by the use of high ionic strength in conbination with low pH buffer to isolate ctDNA from sorghum,wheat, rice and pea. The results show that the improved method has the advantages of higher yield(185-282 μg/100g leaves of sorghum,150-233μg/100g leaves of pea), better quality, and simple manipulation.
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