Cloning and Characterization of Novel Rice Cell cDNAs Under Iron-deficiency by Improved DDRT-PCR Method

An improved differential display reverse transcript PCR(DDRT-PCR) approach was used to identify novel genes treated by iron-deficiency from rice cells.Total eight differential fragments(IDR1-8) were identified.Further northern blotting indicated that five of them were positive;three of those positive fragments were suppressed in iron-deficiency condition,while two of them were induced under iron-deficiency.Sequence analysis revealed that three of them (IDR2,IDR3,IDR8) represent novel genes.Two of them (IDR2,IDR8) were unknown genes,while the IDR3 was homogenous to some known genes by blasting approach.