Tissue Culture and Plant Regeneration from Immature Inflorescence Explants of Chenopodium glaucum L.
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Abstract
A protocol was reported for high frequency adventitious shoot induction and plantlet regeneration from immature inflorescence explants of Chenopodium glaucum.Adventitious shoots were induced from immature inflorescence cultured on MS medium supplemented with 1.0 mg·L-1 6-BA and 0.4 mg·L-1 IBA for 35 days.Induction frequency of adventitious shoots was about 66.7%.Adventitious shoots were further multiplied vigorously and maintained for a long time on the same medium for adventitious shoot induction.Rooting was achieved after adventitious shoot was transplanted to 1/2 MS+NAA 0.2 mg/L medium for 2-3 weeks.
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