Callus Induction and Plantlet Regeneration Systems from a Resources Plant Apocynum venetum L.

To establish the regeneration systems for Apocynum venetum L., leaves, stems, and roots from its aseptic seedlings were cultured in MS mediums with different concentration combinations of NAA and 6-BA. Results showed that both the callus inducement rates from leaves and stems could reach 100% on mediums with several combinations of NAA and 6-BA. When callus were transferred to induce adventitious buds, however, their differentiation rates were quite different. Adventitious buds could be found on callus from stems in several mediums, with the highest differentiation rate of 20%,but could only be found on the callus from leaves in four mediums, with the highest differentiation rate of 35%. The best explants for plantlet regeneration were leaves and the optimum medium for callus inducement and adventitious bud differentiation was MS+NAA 0.1 mg/L+ 6-BA 1.0 mg/L. The optimum root medium was 1/2 MS + NAA 0.2 mg/L, within which the rooting rate could reach 100% and the quality of the root mass was the healthiest. Under suitable hardening and transplanting conditions, the highest survival rate for the regenerated plantlets reached 75%.