Overexpression of <i>OsTTG1</i> affects the epidermal trichome, anthocyanins, and flowering stage in <i>Arabidopsis thaliana</i> (L.) Heynh.

Luo Shuangshuang; Zhang Zongqiong; Nong Baoxuan; Xia Xiuzhong; Liang Shuhui; Liao Zuyu; Feng Rui; Chen Can; Guo Hui; Liang Yuntao; Zeng Yu; Qiu Yongfu; Li Danting; Yang Xinghai

doi:10.11913/PSJ.2095-0837.24071

Plant Science Journal > 2025 > 43(1): 82-91. > DOI: 10.11913/PSJ.2095-0837.24071 CSTR: 32231.14.PSJ.2095-0837.24071

Luo SS，Zhang ZQ，Nong BX，Xia XZ，Liang SH，Liao ZY，Zeng Y，Feng R，Chen C，Guo H，Liang YT，Qiu YF，Li DT，Yang XH. Overexpression of OsTTG1 affects the epidermal trichome, anthocyanins, and flowering stage in Arabidopsis thaliana (L.) Heynh.[J]. Plant Science Journal，2025，43（1）：82−91. DOI: 10.11913/PSJ.2095-0837.24071

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Overexpression of OsTTG1 affects the epidermal trichome, anthocyanins, and flowering stage in Arabidopsis thaliana (L.) Heynh.

Luo Shuangshuang^{1, 2,},
Zhang Zongqiong^2,,
Nong Baoxuan^{2, 3},
Xia Xiuzhong²,
Liang Shuhui²,
Liao Zuyu²,
Feng Rui²,
Chen Can²,
Guo Hui²,
Liang Yuntao²,
Zeng Yu²,
Qiu Yongfu^{1, 3},
Li Danting^{2, 3, ,},
Yang Xinghai^{2, 3, ,}

1.
College of Agriculture, Guangxi University, Nanning 530004, China
2.
Rice Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Nanning 530007, China
3.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Nanning 530004, China

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Received Date: March 24, 2024
Accepted Date: May 15, 2024

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Abstract

Abstract

The OsTTG1 gene encodes a WD40 domain repeat protein, with its knockout in Oryza sativa L. known to affect anthocyanin accumulation. However, whether this gene affects other traits remains unclear. In this study, OsTTG1 was overexpressed in the Arabidopsis thaliana (L.) Heynh. ttg1 mutant. Results showed that overexpression of OsTTG1 (OsTTG1-OE) restored anthocyanin accumulation in leaves and stalks, produced dark brown seeds, and promoted epidermal hair development on leaves, stalks, and calyces. In contrast, the ttg1 mutant exhibited flowering 3–5 d earlier than both the Col-0 and OsTTG1-OE strains, while no significant differences in flowering time were observed between Col-0 and OsTTG1-OE. RNA-seq analysis of differentially expressed genes (DEGs) between OsTTG1-OE and ttg1 at various developmental stages revealed key gene clusters potentially associated with anthocyanin synthesis (MKK9, CYP75B1, TT8, GSTF12, DFR, F3H, CHS, and LDOX), epidermal hair formation (SPL15, TCL1, ETC2, SPL4, SPL13A, ETC3, and GIS), and flowering regulation (FLC, FT, AGL19, HDA5, and LHY). These findings provide an important theoretical basis and reference for understanding the function of OsTTG1 in O. sativa.
- OsTTG1,
- Arabidopsis thaliana,
- Overexpression,
- Epidermal trichome,
- Anthocyanins,
- Flowering stage

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References (45)

References

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Overexpression of OsTTG1 affects the epidermal trichome, anthocyanins, and flowering stage in Arabidopsis thaliana (L.) Heynh.

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