Screening of wild Actinidia germplasms resistant to bacterial canker disease in China
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摘要: 细菌性溃疡病严重威胁猕猴桃(Actinidia)产业发展，本研究对国家猕猴桃资源圃中29个种82份种质资源进行了离体枝条的溃疡病菌接种，以病斑长度评价其抗病能力，将种质划分为高抗至高感7个抗性等级。结果显示：不同种间材料抗性差异显著，大部分种内材料存在明显的抗性分化。82份种质中含高抗种质5份(6.09 %)、中抗种质9份(10.98 %)、低抗种质18份(21.95 %)、耐病种质21份(25.61 %)、低感种质11份(13.41 %)、中感种质9份(10.98 %)及高感种质9份(10.98 %)。32份抗病种质包括软枣(A. arguta (Siebold & Zuccarini) Planchon ex Miquel)6份，异色(A. callosa var. discolor C. F. Liang)5份，黑蕊(A. melanandra Franchet)4份，京梨(A. callosa var. henryi Maximowicz)及毛花(A. eriantha Bentham)各3份，葡萄叶(A. vitifolia C. Y. Wu)、安息香(A. styracifolia C. F. Liang)及紫果(A. arguta (Siebold & Zuccarini) Planchon ex Miquel var. purpurea (Rehd.) C. F. Liang)各2份，阔叶(A. latifolia (Gardner & Champion) Merrill)、红茎(A. rubricaulis Dunn)、山梨(A. rufa (Siebold & Zuccarini) Planchon ex Miquel)、湖北(A. hubeiensis H. M. Sun and R. H. Huang)及黄毛(A. fulvicoma Hance)各1份。本研究结果为猕猴桃抗病品种的杂交选育与创制奠定了基础。Abstract: Bacterial canker disease is a serious threat to the development of the kiwifruit industry. Based on the abundant wild Actinidia germplasm resources in China, screening highly resistant germplasms and breeding resistant varieties with great fruit characters is a primary task for the development of the kiwifruit industry. In this paper, we inoculated isolated branches of 82 germplasms of 29 Actinidia species from the national kiwifruit germplasm nursery with Pseudomonas syringae pv. actinidiae. Disease resistance was evaluated based on lesion length, divided into seven categories from highly resistant to highly susceptible. Results showed distinct differences in resistance among the different species, as well as intraspecific resistance differentiation. Among the 82 germplasms, five were highly resistant (6.09%), nine were moderately resistant (10.98%), 18 were lowly resistant (21.95%), 21 were tolerant (25.61%), 11 were lowly susceptible (13.41%), nine were moderately susceptible (10.98%), and nine were highly susceptible (10.98%). The 32 resistant germplasms included six germplasms of A. arguta (Siebold & Zuccarini) Planchon ex Miquel, five germplasms of A. callosa var. discolor C. F. Liang, four germplasms of A. melanandra Franchet, three germplasms of A. callosa var. henryi Maximowicz and A. eriantha Bentham, respectively, two germplasms of A. vitifolia C. Y. Wu, A. styracifolia C. F. Liang, and A. arguta (Siebold & Zuccarini) Planchon ex Miquel var. purpurea (Rehd.) C. F. Liang, respectively, and one germplasm of A. latifolia (Gardner & Champion) Merrill, A. rubricaulis Dunn, A. rufa (Siebold & Zuccarini) Planchon ex Miquel, A. hubeiensis H. M. Sun and R. H. Huang, and A. fulvicoma Hance, respectively. Thus, this study lays a theoretical foundation for the cross-breeding and creation of disease-resistant varieties of kiwifruit.
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Keywords:
- Actinidia /
- Bacterial canker /
- Germplasm resources /
- In vitro bioassay /
- Resistance evaluation
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[1] 黄宏文. 猕猴桃属:分类资源驯化栽培[M]. 北京:科学出版社, 2013:2. [2] 钟彩虹, 黄文俊, 李大卫, 张琼, 李黎. 世界猕猴桃产业发展及鲜果贸易动态分析[J]. 中国果树, 2021(7):101-108. Zhong CH, Huang WJ, Li DW, Zhang Q, Li L. Dynamic analysis of global kiwifruit industry development and fresh fruit trade[J]. China Fruits, 2021(7):101-108.
[3] McCann HC, Li L, Liu YF, Li DW, Pan H, et al. Origin and evolution of the kiwifruit canker pandemic[J]. Genome Biol Evol, 2017, 9(4):932-944.
[4] 钟彩虹, 李黎, 潘慧, 邓蕾, 陈美艳. 猕猴桃细菌性溃疡病的发生规律及综合防治技术[J]. 中国果树, 2020(1):9-13. Zhong CH, Li L, Pan H, Deng L, Chen MY. Occurrence rule and comprehensive control of kiwifruit bacterial canker disease[J]. China Fruits, 2020(1):9-13.
[5] 裴艳刚, 马利, 岁立云, 崔永亮, 刘晓敏, 龚国淑. 不同猕猴桃品种对溃疡病菌的抗性评价及其利用[J]. 果树学报, 2021, 38(7):1153-1162. Pei YG, Ma L, Sui LY, Cui YL, Liu XM, Gong GS. Resis-tance evaluation and utilization of different kiwifruit cultivars to Pseudomonas syringae pv. actinidiae[J]. Journal of Fruit Science, 2021, 38(7):1153-1162.
[6] Wang FM, Li JW, Ye KY, Gong HJ, Liu PP, et al. Preliminary report on the improved resistance towards Pseudomonas syringae pv. actinidiae of cultivated kiwifruit (Actini-dia chinensis) when grafted onto wild Actinidia guilinensis rootstock in vitro[J]. J Plant Pathol, 2021, 103(1):51-54.
[7] Hoyte S, Reglinski T, Elmer P, Mauchline N, Stannard K, et al. Developing and using bioassays to screen for Psa resistance in New Zealand kiwifruit[J]. Acta Hortic, 2015(1095):171-180.
[8] Lei YH, Jing ZB, Li L. Selection and evaluation of a new kiwifruit rootstock hybrid for bacterial canker resistance[J]. Acta Hortic, 2015(1096):413-420.
[9] Tahir J, Gardiner SE, Bassett H, Chagné D, Deng CH, Gea L. Tolerance to Pseudomonas syringae pv. actinidiae in a kiwifruit breeding parent is conferred by multiple loci[J]. Acta Hortic, 2018(1203):67-70.
[10] Datson P, Nardozza S, Barrett-Manako K, Herrick J, Martinez-Sanchez M, et al. Monitoring the Actinidia germplasm for resistance to Pseudomonas syringae pv. actini-diae[J]. Acta Hortic, 2015(1095):181-184.
[11] Nardozza S, Martinez-Sanchez M, Curtis C, Datson PM, Montefiori M. Screening Actinidia germplasm for different levels of tolerance, or resistance, to Psa (Pseudomonas syringae pv. actinidiae)[J]. Acta Hortic, 2015(1096):351-355.
[12] 石志军, 张慧琴, 肖金平, 杨鲁琼, 孙志伟, 等. 不同猕猴桃品种对溃疡病抗性的评价[J]. 浙江农业学报, 2014, 26(3):752-759. Shi ZJ, Zhang HQ, Xiao JP, Yang LQ, Sun ZW, et al. The resistance evaluation of different kiwifruit varieties to canker[J]. Acta Agriculturae Zhejiangensis, 2014, 26(3):752-759.
[13] 宋雅林, 林苗苗, 钟云鹏, 陈锦永, 齐秀娟, 等. 猕猴桃品种(系)溃疡病抗性鉴定及不同评价指标的相关性分析[J]. 果树学报, 2020, 37(6):900-908. Song YL, Lin MM, Zhong YP, Chen JY, Qi XJ, et al. Evaluation of resistance of kiwifruit varieties (line) against bacterial canker disease and correlation analysis among evaluation indexes[J]. Journal of Fruit Science, 2020, 37(6):900-908.
[14] 温欣, 秦红艳, 艾军, 王月, 韩先焱, 李昌禹. 软枣猕猴桃种质资源溃疡病抗性鉴定方法的建立与评价[J]. 植物保护, 2021, 47(2):193-199. Wen X, Qin HY, Ai J, Wang Y, Han XY, Li CY. Establishment and evaluation of resistance identification method for Pseudomonas syringae pv. actinidiae disease in Actinidia arguta germplasm resources[J]. Plant Protection, 2021, 47(2):193-199.
[15] 刘娟. 猕猴桃溃疡病抗性材料评价及其亲缘关系的ISSR聚类分析[D]. 雅安:四川农业大学, 2015:16-26. [16] 崔丽红, 高小宁, 张迪, 黄丽丽, 黄蔚, 陈继富. 湘西地区猕猴桃细菌性溃疡病抗性资源筛选及其抗性机理研究[J]. 植物保护, 2019, 45(3):158-164. Cui LH, Gao XN, Zhang D, Huang LL, Huang W, Chen JF.Screening of resistance resource and resistance mechanism of kiwifruit to Pseudomonas syringae pv. actini-diae in Xiangxi area[J]. Plant Protection, 2019, 45(3):158-164.
[17] 彭小列, 高建有, 向小奇, 周强, 王发明, 刘世彪. 湘西6种猕猴桃属植物的溃疡病抗性检测与评价[J]. 江西农业, 2021(16):100-102. [18] 王鹏. 猕猴桃抗溃疡病资源的离体筛选及主要类型的分子细胞遗传学分析[D]. 重庆:西南大学, 2019:49. [19] Wang FM, Mo QH, Ye KY, Gong HJ, Qi BB, et al. Evalua-tion of the wild Actinidia germplasm for resistance to Pseudomonas syringae pv. actinidiae[J]. Plant Pathol, 2020, 69(6):979-989.
[20] Wang FM, Li JW, Ye KY, Liu PP, Gong HJ, et al. An in vitro Actinidia bioassay to evaluate the resistance to Pseudomonas syringae pv. actinidiae[J]. Plant Pathol J, 2019, 35(4):372-380.
[21] 王发明, 莫权辉, 叶开玉, 龚弘娟, 蒋桥生, 等. 猕猴桃溃疡病抗性育种研究进展[J]. 广西植物, 2019, 39(12):1729-1738. Wang FM, Mo QH, Ye KY, Gong HJ, Jiang QS, et al. Research progress on kiwifruit resistance breeding to Pseudomonas syringae pv. actinidiae[J]. Guihaia, 2019, 39(12):1729-1738.
[22] 王发明, 齐贝贝, 叶开玉, 龚弘娟, 莫权辉,等. 九个中华类红肉猕猴桃品种的亲缘关系及其溃疡病抗性分析[J]. 分子植物育种, 2021, 19(1):193-199. Wang FM, Qi BB, Ye KY, Gong HJ, Mo QH, et al. The genetic relationship of nine red-fleshed kiwifruit cultivars (Actinidia chinensis) and their resistance to Pseudomonas syringae pv. actinidiae[J]. Molecular Plant Bree-ding, 2021, 19(1):193-199.
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