牡丹染色体的Ag-NORs和Giemsa C带的研究

A STUDY ON Ag-NORs AND GIEMSA C BANDING OF CHROMOSOMES IN Paeonia suffruticosa

摘要: 本文首次报道了紫斑牡丹和栽培牡丹5个重要品种的Ag-NORs和Giemsa C带带型。所有材料的核型组成均为2n=2x=10=6m+2sm+2st。多数材料具6个Ag-NORs,但在染色体上的分布位点不同。C带的差别主要表现在第1、2、3对染色体上。以上两种核学特征,具有品种的特异性。分带所显示的端(T)带和Ag-NORs的数目及分布基本一致。牡丹染色体的端带,本质上是N带;常规染色所显示的所谓随体,实际上是瑞部核仁组成区(NORs)。此外,对牡丹的细胞学研究技术进行了讨论。

Abstract: In this paper, the Ag-NORs and Giemsa C banding patterns of 5 cultivars in Paeonia suffruticosa and P.suffrugioosa var. papaveracea were reported for the first time. All of their karyotypes are 2n=2x=10=6m+2sm+2st. They have 6Ag-NORs which are different in location on chromosomes. The C banding patterns are also varied in 1, 2, 3 pair chromosomes.It was found that the numbers and locations of terminal bands (T) and AgNORs of chromosomes are same Therefore, T bands are actually N bands, and the satellites shown by normal stained are actually terminal NORs.