Studies on Transgenes 1Dx5 and 1Ax1 Transferred into Elite Wheat Cultivars by Cross Breeding
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摘要: 在获得外源品质基因1Dx5和1Ax1超量表达的转基因小麦的基础上,利用小麦转基因品系‘B72-8-11b’和‘B102-1-2’为父本,主要以湖北省栽培品种‘鄂麦12’为母本,配置杂交组合。杂交后代中采用系谱选择法,结合HMW-GS鉴定,研究了转基因小麦外源品质基因在F1、F2、F3、F4代的传递,并筛选出外源1Dx5或1Ax1基因保持超表达的2个新型转基因株系;同时证明了将外源品质基因向栽培品种转育,是提高小麦优质亚基含量和提高HMW-GS总量的有效方法之一。Abstract: For introducing the transgenes from transgenic wheat ’B72-8-11b’ and ’B102-1-2’ into elite wheat lines,two wheat crosses had been made in 2002.In the crosses,elite subunits 1Dx5 and 1Ax1 were over-expressed in the male parents ’B72-8-11b’ and ’B102-1-2’ respectively,while the female parent ’Emai 12’ were selected with better agronomic traits.The expression of exogenous 1Dx5 and 1Ax1 gene in F1,F2,F3 and F4 generations was tested by SDS-PAGE,then the inheritance patten of the transgenes was analysed.Two lines had been selected from the progenies of the crosses in which the subunit 1Dx5 or 1Ax1 is over-expressed and the endogenous HMW-GS compositions are different from each other.The results demonstrated that the introduction of transgenes from transgenic wheat lines into elite varieties by crossing is an effective way to transfer extra HMW-GS gene.
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Keywords:
- Transgenic wheat /
- Over-expression /
- New germplasm
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