花生愈伤组织的诱导和再生体系的建立

详细信息

Callus Induction and Establishment of Efficient Regeneration System of Arachis hypogaea L.

Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi’an 710069, China

摘要: 以花生下胚轴为外植体,MS和1/2 MS为基本培养基,用不同浓度的激素及琼脂对愈伤组织诱导及分化的影响进行了试验,确定MS+6-BA 3.0 mg·L^-1+NAA 0.4 mg·L^-1+0.7%琼脂为诱导愈伤的最佳培养基。提高琼脂浓度可有效抑制玻璃化和褐化的发生,且琼脂浓度越大,愈伤组织越易分化出丛生芽。丛生芽在1/2 MS+NAA1.0 mg·L^-1+0.7%琼脂培养基上生根率最高,达95%以上。
- 花生 /
- 下胚轴 /
- 琼脂浓度 /
- 离体培养
Abstract: The tissue culture of peanut(Arachis hypogaea L.) was studied.The hypocotyls were used as explants on basal MS or 1/2 MS medium supplemented with different hormone combinations and agar.The result showed that the best medium for the inducement of callus was MS+6-BA 3.0 mg·L^-1+NAA 0.4 mg·L^-1+0.7% agar.Increasing of concentration of agar could inhibit vetrification and browning,and make callus easier to differentiate buds.The medium with 1/2 MS+NAA 1.0 mg·L^-1+0.7% agar was favorable to the growth of roots and the rate of rooting was more than 95%.
- Arachis hypogaea L. /
- Hypocotyl /
- Agar concentration /
- Tissue culture