Abstract: Hypericum hubeiense L.H.Wu et D.P.Yang is a new species of Hypericum(Clusiaceae)distributing in China.The qualitative and quantitative analysis of the chemical constituents in the hydroalcoholic extract of H.hubeiense was performed by using HPLC/DAD/ESI-MS.12 main constituents were identified or tentatively identified.The contents of hypericins in the hydroalcoholic extract of H.hubeiense were measured by HPLC analysis monitored at 590 nm using synthetic hypericin as the external standard compound.