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镉对蒜根生长的毒害及抗坏血酸、铁盐的解毒效应

DETOXICATION OF ASCORBIC ACID AND MOLYSITE ON THE ROOT GROWTH OF GARLIC UNDER CADMIUM POLLUTION

  • 摘要: 蒜鳞茎在不同浓度(100~400mg/L)的Cd溶液中处理24~48h后,观察到随着Cd浓度的递增或培养时间的延长,根的生长速率递减或停止,很尖细胞有丝分裂指数下降,分裂细胞异常率增高。分裂细胞异常主要表现为低毒时,染色体的C-有丝分裂、染色体桥以及高毒时,染色体粘连、断裂、核解体等。用抗坏血酸、铁盐进行恢复培养后,根的生长速率和有丝分裂指数均有提高,分裂细胞异常率降低,具有一定的解毒效应。

     

    Abstract: The effects of cadmium solutions of different concentration (100-400 mg/L) on root of garlic were studied.After 24, 48 h of treatment,obvious morphological and cytological changes were observed.With the increase of cadmium concentration or treatment time, the rate of root growth decreased successively or stopped, the mitosis index of root tip cells reduced, the number of anomalous dividing cells rose.The main type of chromosomal aberrations were C-mitosis, chromosome bridge in lower toxicity; fragment, stickiness, fluidization of chromosomes, micronuclei and disintegration of nuclei in higher toxicity.After the roots were toxicated in cadmium solution for 48 h,recovery culture with ascorbic acid, molysite and tap water alleviated the above symptom of toxcity significantly.The rate of root growth and mitosis index were increased.The number of anomalous dividing cells were decreased.The results showed the ascorbic acid and molysite possessed definite detoxication.

     

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