Abstract: The callus growth curves of Asparagus officinalis L.presented "S" types under different light factors but with a similar irradiance.The quantities of soluble proteins of callus were the highest in yellow,in turn in blue,dark,white,green and red lights. The activity of non-specific esterases(NSE)had three peaks in blue, yellow and red lights, but two peaks in green, white and dark lights.The decline of the peak values was in regularorder of yellow,blue, white,red,green, dark lights in the twenty-seventh-day.Except that it had two peaks in red light,the activity of peroxidase(POD)had one peak in the other lights.The decline of the peak values was in regular order of yellow,blue,green,white,dark and red lights.The activity of superoxide dismutase(SOD) presented two peaks in different light factors,and the peak value in white was the highest.