Abstract: The different developmental phases of leaves in Polygonatum cyrtonema and P.anhuiense are studied in this paper.The results are as follows:(1) Stomatal apparatus indexes are both from low to high,when reach to max,then gradually reduce to stabilization.(2) The tendency of length and width ratio of leaf in both P.cyrtonema and P.anhuiense are going up,and which has relativity with leaf shape,but there is no any obvious relativity in length and width ratio of leaf and epidermis cell.(3) The styles of development of leaf epidermis are the same,which are the processes of growth in width and length.(4) In the process of leaf differentiation,the first emergency is epidermis,then mesophyll,at last is vein.(5) The leaf margin of P.cyrtonema have many big parenchyma cells in mesophyll at the early developmental stage,then disappeared or continue existed when leaf mature,but in P.anhuiense,there is no big parenchyma cell appear in all developmental processes.Those studies accumulate experimental data for studies on leaf development of whorled leaf and alternate leaf in Polygonatum Mill.