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吊竹梅叶表皮胞核趋伤性的组织化学观察

HISTOCHEMICAL OBSERVATIONS ON THE TRAUMATAXIS OF EPIDERMAL NUCLEUS OF THE LEAF OF ZEBRINA

  • 摘要: 1.应用细胞学及组织化学的方法,研究了吊竹梅叶表皮在胞核趋伤过程中细胞核、白色体、线粒体、核酸、硷性磷酸酶、硷性ATP酶的变化。2.趋伤胞核较一般胞核的平均直径增长7.7%。在Feulgen反应的制片中。趋伤胞核的染色比正常的核为深,说明DNA含量有所提高。同时,趋伤胞核核仁的哌咯宁染色较正常为深,表明RNA含量也有所增高。3.胞核趋伤现象发生后,白色体和线粒体也随核转移。4.硷性磷酸酶主要分布在白色体中;硷性ATP酶主要分布在线粒体中。胞核趋伤后,这两种酶也随着白色体和线粒体转移,同时,它们的活性也有所增加。5.以上各种变化,加强了物质和能量的供应,对伤口愈合提供了有利的条件。

     

    Abstract: 1. By means of cytological and histochemical methods, the dynasties of nuclei, leucoplasts, mitochondria, nucleic acid, alkaline phosphatase and alkaline adenosine-triphosphatase in the process of traumataxis of the epidermal nuclei of the leaf of Zebrina pendula have been investigated.2. The average diameter of the epidermal nuclei of the leaf of Z. pendula after traumataxis increased about 7.7% in comparison with that of normal nuclei. The slide of Feulgen reaction shows that traumatized nucleus stains deeper than the normal one does. This indicates an increase in DNA content.Meanwhile,the nucleolus of the traumatized nucleus stains deeper in pyroninstain than the normal one does.This indicates an increase in RNA content.3. After the occurrence of the traumataxis of the nuclei, the leucoplasts and mitochondria translocated along with the traumatized nucleus also.4. The alkaline phosphatase was mainly localized in the leucoplasts, and the alkaline adenosine-triphosphatase in the mitochondria.After traumataxis,these two kinds of enzymes translocated along with the leucoplasts and mitochondria. The activities of the enzymes increased at the same time.5. All the changes described above strenthened the supplement of matter and energy, which would be advantageous to the recovery of the wound.

     

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