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蛇床幼茎离体培养中体细胞胚胎形成的观察

OBSERVATIONS ON SOMATIC EMBRYOGENESIS OF CNIDIUM MONNIERI CULTURED IN VITRO

  • 摘要: 蛇床幼茎外植体经诱导产生了愈伤组织。在MS+2,4-D0.2mg/L+ZT0.4mg/L培养基中,愈伤组织转变成胚性愈伤组织。转入MS+NAA0.2mg/L+ZT0.8mg/L培养基以后,胚性愈伤组织分化出体细胞胚胎。体细胞胚胎在MS+NAA0.5mg/L培养基中可直接发育成为完整植株。显微观察表明,体细胞胚胎产生于愈伤组织的表层细胞或内部细胞。在鱼雷胚期已有螺纹导管的分化。子叶期的维管组织从两片子叶伸向胚根,呈“丫”形。

     

    Abstract: Light yellow-green calli were formed from young stem explants of Cnidium monnieri cultured on MS medium containing 2mg/L 2,4-D,0.2mg/L KT and 1000mg/LH.The calli were cultured on MS medi-um supplemented with 0.2mg/L2,4-D,0.4mg/L ZT and 1000mg/LCH,embryogenic calli were obtained.On MS medium supplemented with 0.2mg/L NAA and 0.8mg/L ZT, prolonged culture of the embryogenic calli resulted in embryoids formation.Germination of the embryoids into plantlets was achieved on MS medium added 0.5mg/L NAA.Section of speciments for microscopy showed that the embryoids were originated from the periphery or the interior of embryogenic calli.

     

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