利用电激法转化小麦幼胚的研究

A STUDY ON GENE TRANSFORMATION OF IMMATURE WHEAT EMBRYOS BY ELECTROPORATION

摘要: 利用我们实验室自制的脉冲放电式电激仪HPES－3,我们将合bar基因即PAT酶(phosphinothricin acetyltransferase)基因的pDM302质粒DNA导入了一个春小麦品种(Triticum aestivum L.)"T₂₀₀₃"的幼胚中。经过在含有ppt(phosphinothricin)的选择培养基上筛选,得到了抗ppt的愈伤组织和再生小苗。PCR检测与DNA点杂交结果显示,外源bar基因已整合进了转基因的小麦植株中。
- 小麦 /
- 幼胚 /
- bar基因 /
- 电激 /
- 转基因植株
Abstract: By use of the HPES-3 pulse discharging electroporation system that is developed by our laboratory, we have transferred the bar gene(phosphinothricin acetyltransferase gene) in plasmid pDM302 into the immature embryos of a spring wheat cultivar (Triticum aesivum L.) "T2003".After selection for 2～3 months, we have obtained the ppt (phosphinothricin)-resistant callus, from which regenerated a wheat plantlet.PCR analysis and DNA dot hybridization showed the foreign bar gene had been integrated into the genomes of the transformed wheat plantlet.
- Wheat /
- Immature embryo /
- bar gene /
- Electroporation /
- Transgenic plant