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两种肥力水平下水稻苗高QTL的比较分析

Comparison of QTL for Seedling Height Identified under Two Fertilizer Levels in Rice

  • 摘要: 利用一个来源于粳/籼交组合的水稻重组自交系群体进行盆栽试验,设正常肥力(对照CK)和低肥力(不施肥)2个处理,分别在播种后25d(时期Ⅰ)和50d(时期Ⅱ)取样测定秧苗的苗高。结合一张含有198个标记的高密度分子遗传图谱,对性状进行复合区间作图。共检测到8个水稻苗高QTL,分别位于第1、3、5、6、8和10号染色体上,各QTL对性状的贡献率为4%~12%。通过对2种肥力水平下水稻苗高QTL的比较分析,发现大多数QTL只在1种肥力水平下表达,QTL与不同肥力水平之间存在着显著的互作。唯一一个在2种肥力水平下均能稳定起作用、而且加性效应的方向一致的QTL是qSH-3-2,该QTL位于3号染色体标记区间RM156-RM16,其加性效应值为正,增效基因来自于亲本Lemont。此外,有3个QTL(qSH-1、qSH-3-3qSH-5)在2个抽样时期均起作用,且加性效应的方向一致。对利用分子标记辅助选择改良水稻品种的耐低肥特性的育种策略进行了讨论。

     

    Abstract: A recombinant inbred line population of rice(Oryza sativa L.)was directly seeded in plastic pots in a randomized complete block with two treatments,control(normal fertilizer level)and no fertilizer.Seedling height was measured at stages Ⅰ(25 days after seeding)and Ⅱ(50 days after seeding),respectively.Composite interval mapping was carried out using a high density linkage map based on the same population,leading to the identification of 8 main-effect QTL for seedling height,each explaining 4%-12% of the phenotypic variation.Comparing the QTL identified under two fertilizer levels resulted in a finding that most of the QTL showed effects on the trait only under one fertilizer level,indicating presence of significant QTL by fertilizer level interactions.Only one QTL(qSH-3-2)was found to produce consistent additive effect on the trait under two fertilizer levels.This QTL was located to the interval RM156-RM16 of chromosome 3 and its positive allele contributed by the parent Lemont.In addition.There were three QTL(qSH-1,qSH-3-3 and qSH-5)showing consistent additive effects at stages Ⅰ and Ⅱ.Breeding strategies for improvement of tolerance to low fertilizer stress in rice via marker-aided selection were discussed.

     

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