Abstract:
The effects of different storage temperatures on lignification and related enzyme activities of loquat(
Eriobotrya japonica Lindl.cv.Jiefangzhong) pulp stored at 4℃ and 12℃ were studied. The results showed that phenylalanine ammonia lyase(PAL,EC 4.1.3.5.) activity, 4-coumarate coenzyme A ligase(4-CL,EC 6.2.1.12.) activity,cinnamyl alcohol dehydrogenase(CAD,EC 1.1.1.195.) activity and peroxidase(POD,EC 1.11.1.7.) activity of loquat pulp stored at 4℃ were higher than those stored at 12℃,while polyphenol oxidase(PPO,EC 1.10.3.2.) activity was lower.The firmness and lignin content of loquat pulp at 4℃ increased during storage,which results in comparatively serious lignification in loquat pulp.The firmness and lignin content of loquat pulp at 12℃ were lower than at 4℃,hence a lo-wer level of lignification in loquat pulp.The main factor for lignification in loquat pulp is that the low temperature caused higher phenylalanine ammonia lyase activity and peroxidase activity.