水稻抗白叶枯病菌毒素细胞无性变异系的初步筛选

详细信息

A PRELIMINARY SCREENING OF SOMACLONAL VARIANTS OF RICE (ORYZA SATIVAL.)RESISTANT TO XAMTHOMONAS ORYZAE DOWSON

摘要: 应用湖北水稻白叶枯病强毒菌株“江陵691”活细菌、细菌培养滤液、病菌粘液多糖和菌体多糖。对水稻粳105、粳3037和科选1号的幼穗及花药所形成的愈伤组织进行共培养筛选。获得体细胞再生植株443株,花粉植株31株,其中17株为单倍体(n=12).同工酶谱显示,筛选出的粳105愈伤组织的过氧化物酶同工酶活性加强,带数增多。
- 水稻 /
- 白叶枯病 /
- 细胞无性变异系
Abstract: Xanthomonas oryzoe Dowson ssp.Jiangling 691 strain with strong virulence and its extracts,toxin were used as selective agent to screen variants of rice (Oryza sativa L.cv.Wuangeng 105,Wuangeng 3037 and Kexuanyihao)which are resistant to Xanthomonas oryzae Dowson.443 somatic regenerations and 31 pollen regenerations were obtained.of 31 pollen regenerations,17 green plantlets were confirmed as haploid (n =12)by chromosome examination and therefore they were chromosome-doubled.Isozy me patterns showed that activity and bands of peroxidase isozyme of callus selected were increased.
- Rice(Oryza sativa L.) /
- Xanthomonas oryzae Dowson /
- Somaclonal variants