Abstract: Proteomics which is the new discipline in the time of the post-genomics develops rapidly in the life science.Techniques of separation and detection in proteomics are the important foundation in promoting the development of proteomics.The present paper has documented the current situation and new development of the techniques of separation and detection in this area,including 2-dimensional gel electrophoresis,chromatography and mass spectrometry.It also summarized our lab’s work on proteomics assisted by bioinformatics.