长日照处理对牵牛开花抑制作用研究　Ⅱ.光周期处理与牵牛花芽分化及基因活动的关系

STUDY ON THE INHIBITORY EFFECT OF LONGDAY TREATMENT ON PHARBITIS NIL Ⅱ.THE RELATION BETWEEN DIFFERENT DARK LENGTH PHOTOPERIODS AND FLOWER BUD DIFFERENTIATION AND GENE EXPRESSION IN THE COTYLEDONS

摘要: 单个光周期暗期长度短于12h时,牵牛植株营养生长旺盛,开花受到抑制,并且出现了诱导光周期处理(ISD)子叶中没有的二种蛋白质或多肽(pI4.1,MW16.5kD;pI4.2,MW16.5kD)。连续光照处理(ICL)子叶内出现了短日照处理(ISD)子叶内没有的体外翻译蛋白质分子量为17.4kD的Poly(A⁺)mRNA。牵牛子叶内的这些变化可能与抑制牵牛花芽分化有一定的关系。

Abstract: The growth of Pharbitis nil was promoted and its flowering was inhibitedwhen the dark length of single photoperiod was less than₁₂ h.The results of 2-SDS-PAGE indicated that two kinds of proteins (pI4.1, MW16.5 kD; PI4.2, MW16.5 kD)existed in cotyledons.These proteins were disappeared in the cotyledon treated with induced photoperiod when the light length was over 12 h.Poly(A⁺)mRNA in the cotyledons with continuous light treatment (l CL) whose protein weight in vitro translationwas 17.4 kD was not discovered in the one treated with induced photoperiod (lSD).These changes in the Pharbitis nil cotyledons might be related to inhibiting the flowerbud differentiation.